By G. Aumüller, P. M. Holterhus, W. Eicheler (auth.), Martin Tenniswood, Horst Michna (eds.)
It is now generally authorized that cells be ready to start up a software of gene-directed demise, a strategy referred to as "apoptosis" which can even be used as an leading edge process for healing intervention in melanoma remedy. specialists during this zone document at the law and keep an eye on of apoptosis in hormone established (neoplastic) tissue and the prospect to take advantage of energetic telephone loss of life for healing application.
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Extra info for Apoptosis in Hormone-Dependent Cancers
Cancer Res 52:4313-4319 Colombe! M, Symmans F, Gil S, O'Toole KM, Chopin D, Benson M, Olsson CA, Korsmeyer S, Buttyan R (1993) Detection of the apoptosis-suppressing oncoprotein bcl-2 in hormone-refractory human prostate cancers. Am J Pathol 143:390-400 di Sant'Agnese PA (1992) Neuroendocrine differentiation in carcinoma of the prostate. Cancer [Suppl] 70:254-268 English HF, Kyprianou N, Isaacs JT (1989) Relationship between DNA fragmentation and apoptosis in the programmed cell death in the rat prostate following castration.
Apoptosis in the Prostate ................................. Material and Methods . . . . . . . . . . . . . . . . . . Tumor Transplantation . . . . . . . . . . . . . . . . . . Hormonal Manipulation . . . . . . . . . . . . . . . . . Immunohistochemistry . . . . . . . . . . . . . . . . . . LNCaP Cell Culture . . . . . . . . . . . . . . . . . . . Detection of Apoptosis in LNCaP Cell Cultures . . . . . .
Focal areas with TGase- and DNase-immunoreactive cells were encountered. In prostate cancer (three specimens), divergent results were found in that both strongly positive cells (for either of the antigens studied) and several negative cells were present. The immunoreaction pattern was clearly different from the normal state and will be described elsewhere. The number of terminal transferase-reactive nuclei was elevated compared to the intact glands both in BPH and prostatic carcinoma. As only few specimens have been studied as yet, no deductions are possible from these preliminary observations.